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Expert consensus regarding standardization of sample preparation for clotting time assays

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Abstract

Accurate clotting time assay results are vital, as the test is employed to indicate the amount of oral anticoagulant to be prescribed, while it is also used for screening the hemorrhagic and thrombotic diseases. The procedure chosen for preparation of a patient blood sample including centrifugation can contribute to significant differences in the results obtained. Thus, for the purpose of proposing a standardized method to appropriately prepare blood samples prior to assay, the Japanese Society of Laboratory Hematology organized the Working Group for Standardization of Sample Preparation for Clotting Time Assays (WG). Following reviews of previously announced guidelines and original experimental results, consensus was obtained by the WG, with the main findings as follows. (1) The recommended anticoagulant in the blood collection tube is sodium citrate solution at 0.105–0.109 M (3.13–3.2%). (2) Whole blood samples should be stored at room temperature (18–25 ˚C) within 1 h of collection from the patient. (3) For plasma preparation, centrifugation at 1500 × g should be performed for at least 15 min or at 2000 × g for at least 10 min at room temperature. (4) After the plasma sample is prepared, it should be stored at room temperature and assayed within 4 h.

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Acknowledgements

We thank Hiroko Juraku and Kyoko Kumagai for helping with the study on the influences of residual platelets on clotting time assays. We also thank Yayoi Ishida for the administrative support in writing the manuscript. Prior to submitting the manuscript, Intermed English Services was asked to proofread the text for language usage. The working group for standardization of sample preparation for clotting time assays, Japanese society for laboratory hematology are, Masahiro Ieko, Yutaka Komiyama, Satoshi Yamazaki, Hisako Katagiri, Chisato Shimazu, Sumiyoshi Naito, Masato Matsuda, Yoichi Yuki, Kazuyoshi Miyata, Rika Arai, Osamu Kumano, Mari Emmi, Tatsuya Yoshida, Hideaki Tanaka, Toshihiko Okuhara, Tetsuji Yamashita, Yasuji Sekine, Masaru Homma, Yohko Kawai, Kaoru Tohyama.

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Correspondence to Masahiro Ieko.

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K. Miyata is an employee of Nippon Becton Dickinson Company, Ltd., Japan. R. Arai is an employee of Terumo Corporation, Japan. O. Kumano is an employee of Sysmex Corporation, Japan. M. Emmi is an employee of Sekisui Medical Co. Ltd., Japan. T. Yoshida and T. Yamashita are employees of LSI Medience Corporation, Japan. H. Tanaka is an employee of Fujirebio Inc., Japan. T. Okuhara is an employee of Fukuyama Clinical Laboratory, Japan. Y. Sekine is employee of the Kohtohbiken Medical Laboratories, Japan. The other authors have no conflicts of interest to declare.

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Ieko, M., Komiyama, Y., Yamazaki, S. et al. Expert consensus regarding standardization of sample preparation for clotting time assays. Int J Hematol 112, 614–620 (2020). https://doi.org/10.1007/s12185-020-02983-x

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  • DOI: https://doi.org/10.1007/s12185-020-02983-x

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