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タイトル: | The Japanese Society of Pathology Guidelines on the handling of pathological tissue samples for genomic research: Standard operating procedures based on empirical analyses |
著者: | Kanai, Yae Nishihara, Hiroshi Miyagi, Yohei Tsuruyama, Tatsuhiro Taguchi, Kenichi Katoh, Hiroto Takeuchi, Tomoyo Gotoh, Masahiro Kuramoto, Junko Arai, Eri Ojima, Hidenori Shibuya, Ayako Yoshida, Teruhiko Akahane, Toshiaki Kasajima, Rika Morita, Kei-ichi Inazawa, Johji Sasaki, Takeshi Fukayama, Masashi Oda, Yoshinao |
キーワード: | biobank empirical analysis genome research pathological tissue sample sample collection and strogage |
発行日: | Feb-2018 |
出版者: | Wiley-Blackwell |
誌名: | Pathology International |
巻: | 68 |
号: | 2 |
開始ページ: | 63 |
終了ページ: | 90 |
抄録: | Genome research using appropriately collected pathological tissue samples is expected to yield breakthroughs in the development of biomarkers and identification of therapeutic targets for diseases such as cancers. In this connection, the Japanese Society of Pathology (JSP) has developed “The JSP Guidelines on the Handling of Pathological Tissue Samples for Genomic Research” based on an abundance of data from empirical analyses of tissue samples collected and stored under various conditions. Tissue samples should be collected from appropriate sites within surgically resected specimens, without disturbing the features on which pathological diagnosis is based, while avoiding bleeding or necrotic foci. They should be collected as soon as possible after resection: at the latest within about 3 h of storage at 4°C. Preferably, snap‐frozen samples should be stored in liquid nitrogen (about −180°C) until use. When intending to use genomic DNA extracted from formalin‐fixed paraffin‐embedded tissue, 10% neutral buffered formalin should be used. Insufficient fixation and overfixation must both be avoided. We hope that pathologists, clinicians, clinical laboratory technicians and biobank operators will come to master the handling of pathological tissue samples based on the standard operating procedures in these Guidelines to yield results that will assist in the realization of genomic medicine. |
著作権等: | © 2018 The Authors. Pathology International published by Japanese Society of Pathology and John Wiley & Sons Australia, Ltd. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
URI: | http://hdl.handle.net/2433/230577 |
DOI(出版社版): | 10.1111/pin.12631 |
PubMed ID: | 29431262 |
出現コレクション: | 学術雑誌掲載論文等 |
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